Journal: iScience
Article Title: An antibiotic derivative as a new potential tool in the prevention of hemolytic uremic syndrome
doi: 10.1016/j.isci.2025.113076
Figure Lengend Snippet: Quantitative capillary western blot analysis of the proteins derived from human EVs Human EVs were isolated after treatment of human blood with vehicle or with 2 nM Stx2a in the absence or in the presence of 0.01 μg/mL NAB815 and their proteins extracted as described in . (A) Representative WES of the analyzed antigens (Alix, CD45, and CD42a) and associated proteins (Stx2a) is shown. (B–E) Data obtained with different human donors ( n = 4) represent the percentage (mean ± SD) of the quantitative determinations of the different antigens with respect to controls (C–E) or Stx2a (B). (F–H) Data obtained with different human donors ( n = 4) are expressed as percentage (mean ± SD) of the stimulation in the presence of Stx2a that was set at 100%. Different primary antibodies were used: rabbit polyclonal anti-Alix 1:50 (Novus Biological) as an EV marker, mouse monoclonal anti-CD45 1:250 (BD Transduction Laboratories) as a leukocyte marker, rabbit polyclonal anti-CD42a 1:10 (GeneTex) as a platelet marker, and rabbit polyclonal anti-Stx2a 1:50 (Dr. Stefano Morabito, ISS Rome) to detect the toxin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (two-tailed unpaired t test). See also .
Article Snippet: Different primary antibodies were used: rabbit polyclonal anti-Alix 1:50 (Novus Biological) as an EV marker, mouse monoclonal anti-CD45 1:250 (BD Transduction Laboratories) as a leukocyte marker, rabbit polyclonal anti-CD42a 1:10 (GeneTex) as a platelet marker, and rabbit polyclonal anti-Stx2a 1:50 (Dr. Stefano Morabito, ISS Rome) to detect the toxin.
Techniques: Western Blot, Derivative Assay, Isolation, Marker, Transduction, Two Tailed Test
